RAMOS CARS 3D Scanning Spectrometer
Confocal Raman Solutions
Description
RAMOS CARS is the multifunctional multi-channel 3D scanning laser microscope-spectrometer. RAMOS CARS is intended for simultaneous and multifunctional analysis: high-speed imaging by CARS signal; confocal Raman imaging; confocal fluorescence imaging; confocal imaging in the reflected laser radiation; high-contrast imaging in the transmitted laser radiation; as well as visualization of a surface profile by means of the second harmonics generation signal. Microscope RAMOS CARS combines in one system: - CARS scanning microscope - Raman/luminescent scanning confocal microscope - Conventional scanning confocal laser microscope Applications: - Nanobiotechnology: real-time noninvasive analysis of biological samples (cells and components of living cells) with high spatial resolution. - Micro- and nanotechnology investigations of properties of non-biological microstructures: semiconductors, liquid crystals, polymers, pharmaceutical components, micro- and nanoparticles.
- Scientific research equipment
- Laser Spectrometer
- Scanning Spectrometer
Product characteristics
- Spatial XYZ resolution of CARS signals (Objective lens: 60х, NA=1.2, water immersion)
- < 0.7 μm
- Spatial resolution of Raman signals
- XY< 300 nm, Z< 700 nm
- Spectral detectable range
- CARS signals: 985 – 5000 cm-1, Raman signals: 75 – 6000 cm-1
- Spectral resolution of CARS signals
- 7-8 cm-1
- Spectral resolution of Raman signals
- 0.25 cm-1(grating 75 l/mm Echelle); 0.6 cm-1 (grating 1800 g/mm)
- Scanning range (fast scanning mode, 60x lens)
- XY: 225 х 225 μm, Z: 80 μm
- Control and automation
- Fully motorized
- Optical microscope type
- inverted
- Optical microscope model
- Nikon Ti-U
- Sample stage
- motorized
- - travel range
- 114 х 75 mm
- - accuracy (for 1 mm traveling)
- 0.06 μm
- - XY repeatability
- ± 1 μm
- - minimal step
- 0.02 μm
- Micro-objective lens
- 60 х NA-1.2 water immersion, 20 x NA-0.45
- Z-scanner type
- piezo scanner
- Z-scanner lens movement range
- 80 μm
- Z-scanner minimal step
- 50 nm
- Z-scanner repeatability
- < 6 nm
- Illuminator for reflection mode
- Halogen lamp 100 W
- Illuminator for transmission mode
- LED
- Laser input port
- motorized triple turret
- Detector (Raman, E-CARS, fast measurements)
- Hamamatsu Photosensor module H7844 (with TE cooling)
- Scanners
- Galvano-mirror scanners (XY)
- Scanning Model
- raster high speed and start-stop mode
- Scanning area
- 225 μm х 225 μm (objective lens 60Х)
- Full frame scanning speed
- 4 s/frame: 1000 х 1000 points
- Confocal pinhole
- motorized confocal pinhole, continuously adjustable from 0 to 1.5 mm
- Number of registration channels
- 5
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